SURVIVAL AND CYCLE-PROGRESSION DELAY OF CULTURED HUMAN LYMPHOMA-CELLS TREATED WITH 1-PROPANOL, 3,3'-IMINODISULFONATE, DIMETHANESULFONATE (ESTER), HYDROCHLORIDE (YOSHI 864)

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  • 1 January 1976
    • journal article
    • research article
    • Vol. 60  (11) , 1637-1645
Abstract
Asynchronous human lymphoma cells treated for 1 h with increasing concentrations of 1-propanol, 3,3''-iminodi-, dimethanesulfonate (ester), hydrochloride (Yoshi 864) revealed a shouldered survival curve typical of the effects of alkylating agents and ionizing radiation on this cell line. Yoshi 864 was unstable under the conditions of treatment, its killing effect being reduced by 50% after only 4 h. Synchronized cells showed stage dependent sensitivity. Early S late G2 and late G1 phases were the most sensitive while mid S and late S and early G2 phases were relatively insensitive. Yoshi 864 induced a concentration and incubation time dependent delay in the transit of asynchronous cells through the G2 phase, with maximum accumulation values obtained after 12 h of incubation with 100 .mu.g/ml. This effect was reversible and no further kinetic changes were noted in the progeny of treated cells. Incubation of synchronized cells for 1 h with 100 .mu.g/ml demonstrated a block in G2, the manifestation of which during the lifespan of the treated cell or in its immediate progeny was cell cycle dependent. Cells treated in G1, early S and mid S phases showed a delay in the subsequent G2 phase of the immediate progeny. There was no correlation between this blocking effect in G2 with cell survival assessed by colony formation. Yoshi 864, although a rather inefficient killing drug, may represent a useful chemical synchronizing agent.