Crude‐tissue‐extract analysis by micro‐electrophoresis in cylindrical continuous polyacrylamide gradient gels
- 1 February 1982
- journal article
- research article
- Published by Wiley in Electrophoresis
- Vol. 3 (6) , 337-341
- https://doi.org/10.1002/elps.1150030608
Abstract
Continuous gradient gels in capillaries with acrylamide concentrations ranging from 2–20 % or alternatively from 2–40 % are equally suited for the fractionation of complex mixtures of macromolecules in aqueous solutions within a range of molecular weights from 103‐106. A time‐saving method for the simultaneous preparation of up to 48 identical linear micro‐gradient polyacrylamide gels is described. With this method it was possible to compare the patterns of aqueous extracts and to determine tissue‐specific bands of the mossFunaria hygrometrica L. Sibth.in the low molecular weight range for the first time.This publication has 23 references indexed in Scilit:
- Miniature ultrathin‐layer isoelectric focusing in 20–50 μm polyacrylamide gelsElectrophoresis, 1981
- A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye bindingAnalytical Biochemistry, 1976
- Isoelektrische Fokussierung in Mikrogelen zur Fraktionierung komplexer Proteingemische im NanogrammbereichHoppe-Seyler´s Zeitschrift Für Physiologische Chemie, 1976
- Mikroelektrophorese in kontinuierlichen Polyacrylamid-Gradientengelen, IV. Der Einfluß von Reduktionsmitteln auf die Proteinelektrophorese in Dodecylsulfat-PuffersystemenHoppe-Seyler´s Zeitschrift Für Physiologische Chemie, 1975
- Mikroelektrophorese in kontinuierlichen Polyacrylamid- Gradientengelen, III[1,2]. Extraktion und Fraktionierung von Ribonucleinsäuren im MikromaßstabHoppe-Seyler´s Zeitschrift Für Physiologische Chemie, 1974
- Micro-Electrophoresis in Continuous-Polyacrylamide-Gradient Gels, II. Fractionation and Dissociation of Sodium Dodecylsulfate Protein ComplexesHoppe-Seyler´s Zeitschrift Für Physiologische Chemie, 1974
- The large scale preparation of chromatographic grade hydroxylapatite and its application in protein separation proceduresJournal of Chemical Technology & Biotechnology, 1973
- Mikroelektrophorese an kontinuierlichen Polyacrylamid-Gradienten-gelen, I. Herstellung und Eigenschaften von Gelgradienten in Kapillaren; ihre Anwendung zur Proteinfraktionierung und MolekulargewichtsbestimmungHoppe-Seyler´s Zeitschrift Für Physiologische Chemie, 1973
- Microelectrofocusing of proteins in capillary gelsBiochemical and Biophysical Research Communications, 1972
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970