MEDIATION OF GUINEA-PIG GASTRIN SECRETION INVITRO BY CYCLIC NUCLEOTIDES

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  • 1 January 1977
    • journal article
    • research article
    • Vol. 73  (1) , 79-83
Abstract
The effect of methacholine, theophylline and cyclic[c]adenylate and guanylate nucleotides on gastrin secretion from antral and proximal duodenal mucosa of the guinea pig was studied. Both 2 mM dibutyryl (db) cAMP and 5 mM theophylline significantly increased gastrin secretion, 4.3 .+-. 0.7 (P < 0.001) and 9.3 .+-. 2.4 pg mg-1 min-1 (P < 0.005), respectively, above basal gastrin secretion (1.5 .+-. 0.4 pg mg-1 min-1). The combined effect of the 2 agents was additive (14.5 .+-. 3.6 pg mg-1 min-1). Db cGMP (2 mM) had no effect on gastrin secretion. Methacholine produced a dose-related increase in gastrin secretion which at maximum equaled the combined effect of theophylline and db cAMP. Gastrin secretion is probably mediated in part by intracellular cAMP but a cooperative involvement of cGMP is not excluded.

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