A New System for Three-Dimensional Tracking of Motile Microorganisms
Open Access
- 1 May 2000
- journal article
- Published by American Society for Microbiology in Applied and Environmental Microbiology
- Vol. 66 (5) , 2238-2242
- https://doi.org/10.1128/aem.66.5.2238-2242.2000
Abstract
A new three-dimensional (3D)-tracking system with optimized dark-field illumination is presented. It allows simultaneous 3D tracking of several free-swimming microorganisms with diameters of >10 μm. Resolution limits and illumination efficiencies for different size classes of microorganisms are treated analytically. First applications for 3D tracking of protists are demonstrated.Keywords
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