INDUCTION OF A FURROW-LIKE DENT ON THE SURFACE OF THE NEWT EGG BEFORE THE ONSET OF CLEAVAGE*

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Abstract
In the amphibian egg a furrow-inducing cytoplasmic component (FIC) was localized along the cleavage furrow which induced a furrow on the polar surface of the cleaving egg under which FIC was injected. This procedure failed on the surface of the uncleaved fertilized egg. A cleavage furrow was induced on the surface of the uncleaved egg of the newt, Cynops pyrrhogaster. A piece of the cortex was cut from the uncleaved egg, which was transplanted to the egg just before or just after the onset of the cleavage, using a fine glass needle. After the transplantation FIC was injected beneath the graft with a capillary. The graft reacted to FIC and a furrow-like dent was induced at the position. Stiffness of the graft increased during the cleavage of the host egg. In contrast to the cortical grafting, a large amount of the cytoplasm excluding FIC was injected under the cortex of an uncleaved egg. After several minutes FIC was deposited at the site. A furrow-like dent was formed in many cases.

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