Effect of Biotin on the Bacterial Formation of Glutamic Acid

1 August 1962

journal article
research article
Published by Oxford University Press (OUP) in The Journal of Biochemistry

Vol. 52 (2) , 108-116
https://doi.org/10.1093/oxfordjournals.jbchem.a127578

Abstract

Cells of Brevibacterium flavum in biotin-rich medium completely oxidize glucose and pyruvate to CO2. Biotin-deprived cells metabolize glucose to CO2 and [alpha]-ketoglutarate. Cells in biotin-rich medium accumulate L-glutamate when detergents (cetyltrimethylam-monium bromide, dodecylsulfate or deoxycholate) are added to reaction mixture. These detergents may make cell membrane permeable to glutamate.

Keywords

BIOTIN/PHARMACOLOGY
BREVIBACTERIUM/METABOLISM
GLUCOSE/METABOLISM
GLUTAMATES/METABOLISM