Biological Properties of Lipid A from Shigella sonnei

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Abstract
Lipid A preparations isolated from Shigella sonnei lipoplysacharides after mild acid hydrolysis were used as immunogens in rabbits. Titers of antisera to lipid A amounted to about 1:640 in passive hemolysis test. Antibodies to lipid A were purified on the column packed with lipid A coupled to Sepharose 4 B. Pure antibodies to lipid A examined in immunoelectrophoresis proved to be immunoglobulins G.The specificity of antisera to lipid A was confirmed in passive hemolysis inhibition test elaborated in this work. From the results of the examination of various products of lipid A degradation in inhibition studies of lipid A—anti‐(lipid A) system, it can be concluded that amino groups of glucosaminyl units substituted by 3‐hydroxymyristoyl residues are immunodominants of lipid A.Lipid A preparations from Shigella sonnei showed distinct anticomplementary activities which disappeared from the examined materials after removal of ester‐linked fatty acids. Shigella sonnei lipopolysaccharides, at suitable concentrations, inhibited also complement fixation. Phase II lipopolysaccharide was the best inhibitor.

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