Fluorometric Assay of Alcohols Using Alcohol Oxidase
- 1 January 1969
- journal article
- research article
- Published by Taylor & Francis in Analytical Letters
- Vol. 2 (1) , 41-48
- https://doi.org/10.1080/00032716908051270
Abstract
Fluorometric methods are described for the assay of ethanol, methanol, allyl alcohol and n-propanol using the enzyme alcohol oxidase. The enzyme catalyzes the oxidation of the alcohols to aldehydes with concomitant formation of hydrogen peroxide. The hydrogen peroxide formed is measured fluorometrically using p-hydroxy-phenylacetic acid and peroxidase. From 0.1–100 μg/ml of the alcohols and from 0.0002 to 0.050 unit/ml of alcohol oxidase can be assayed in 2 minutes with a precision of about ± 2%.Keywords
This publication has 2 references indexed in Scilit:
- Substrates for the fluorometric determination of oxidative enzymesAnalytical Chemistry, 1968
- Alcohol oxidase, a novel enzyme from a basidiomyceteBiochemical and Biophysical Research Communications, 1965