Seedlings from Undeveloped Ovules of Ripe Fruits of Polyembryonic Citrus Cultivars1
Open Access
- 1 June 1980
- journal article
- Published by American Society for Horticultural Science in HortScience
- Vol. 15 (3) , 296-297
- https://doi.org/10.21273/hortsci.15.3.296
Abstract
Embryos, pseudobulbils, and embryogenic callus were obtained by in vitro culture of undeveloped ovules excised from ripe fruits of the navel orange group (C. sinensis [L.] Osbeck) and lemon (C. limon [L.] Burm. f.). Initial culturing was on Murashige & Skoog (MS) medium in 1% agar. Large numbers of seedlings were produced by subculturing the embryos, pseudobulbils, and embryogenic callus on fresh MS medium with added 6-methylaminopurine.Keywords
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