Procaine inhibits cyclic AMP-induced steroidogenesis in isolated bovine adrenocortical cells.
- 1 January 1990
- journal article
- research article
- Published by Elsevier in The Japanese Journal of Pharmacology
- Vol. 52 (1) , 81-85
- https://doi.org/10.1254/jjp.52.81
Abstract
Effects of procaine on dibutyryl adenosine 3'',5''-cyclic monophosphate ((Bu)2cAMP)-, Ca2+- or forskolin-induced steroidogenesis were examined in isolated bovine adrenocortical cells. Procaine (< 1.0 mM) caused a marked suppression of (Bu)2cAMP- or forskolin-induced steroidogenesis in the absence of extracellular Ca2+, but did not affect on Ca2+-induced steroidogenesis in the cells.(Bu)2cAMP decreased the cell associated 45Ca2+. However, procaine (300 .mu.M) inhibited this effect of (Bu)2cAMP. These results suggest that procaine may abolish (Bu)2cAMP-induced Ca2+ release from intracellular calcium store(s) and inhibits steroidogenesis.Keywords
This publication has 11 references indexed in Scilit:
- A possible regulatory role of Ca++-calmodulin system in cellular cholesterol ester hydrolysis in the steroidogenic response to ACTH in bovine adrenocortical cells.Endocrinologia Japonica, 1983
- Forskolin potentiates adrenocorticotropin-induced cyclic AMP production and steroidogenesis in isolated rat adrenal cellsLife Sciences, 1982
- Phospholipid turnover as a possible transmembrane signal for protein phosphorylation during human platelet activation by thrombinBiochemical and Biophysical Research Communications, 1980
- Inhibitory action of chlorpromazine, dibucaine, and other phospholipid-interacting drugs on calcium-activated, phospholipid-dependent protein kinase.Journal of Biological Chemistry, 1980
- Adrenocorticotropin action in isolated adrenal cells. The intermediate role of cyclic AMP in stimulation of corticosterone synthesis.Journal of Biological Chemistry, 1979
- Calcium ion as "second messenger" in corticoidogenic action of ACTH.Endocrinologia Japonica, 1979
- Studies on adrenocorticotropic hormone receptor using isolated rat adrenocortical cells.Endocrinologia Japonica, 1978
- On the role of calcium as second messenger in liver for the hormonally induced activation of glycogen phosphorylaseBiochimica et Biophysica Acta (BBA) - General Subjects, 1977
- Metabolic Regulation of Steroidogenesis in Isolated Adrenal Cells of RatEuropean Journal of Biochemistry, 1976
- Practical Procedure for Estimation of Corticosterone or HydrocortisoneClinical Chemistry, 1958