Biosynthesis of ?-ascorbic acid (vitamin C) by Saccharomyces cerevisiae

Publisher Website
Google Scholar
Abstract
Saccharomyces cerevisiae cells incubated with D -glucose ( D -Glc), D -galactose or D -mannose ( D -Man) synthesised D -erythroascorbic acid ( D -EAA) but not L -ascorbic acid ( L -AA). Accumulation of D -EAA was observed in cells incubated with D -arabinose ( D -Ara) whilst accumulation of L -AA occurred in cells incubated with L -galactose ( L -Gal), L -galactono-1,4-lactone and L -gulono-1,4-lactone. When S. cerevisiae cells were incubated with D -[U- 14 C]Glc, D -[U- 14 C]Man or L -[1- 14 C]Gal, incorporation of radioactivity into L -AA was observed only with L -[1- 14 C]Gal. Pre-incubation of yeast cells with D -Ara substantially reduced the incorporation of L -[1- 14 C]Gal into L -AA. Our results indicate that, under appropriate conditions, yeast cells can synthesise L -AA via the pathway naturally used for D -EAA biosynthesis.

This publication has 0 references indexed in Scilit: