Characterization of an atypical Aeromonas salmonicida strain causing epizootic ulcer disease in cultured eel.

Abstract
A comparison of the biochemical and serological characteristics of atypical Aeromonas salmonicida strains isolated from eel (Anguilla japonica), goldfish (Carrassius auratus), and reference strains of A. salmonicida were reinvestigated.The biochemical and serological properties of causative agents of ulcer disease in eel at Tokushima and Shizuoka prefecture, and Hiroshima University in Japan were similar with isolates previously described. Several biochemical characteristics distinguished the isolates reported here from atypical A. salmonicida strains from goldfish, eel, and typical A. salmonicida.The DNA base composition (mol% guanine plus cytosine) of the isolate was similar with those of some atypical and typical A. salmonicida previously described.The thermostable antigenic structure of atypical and typical A. salmonicida were similar to the isolate reported here. The thermolabile antigenic structures showed some differences among the strains.The structure of lipopolysaccharide (LPS) from all strains demonstrated identical pattern by electrophoretic analysis.

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