Role of glutathione in the prevention of cataractogenesis in rat lenses
- 1 January 1982
- journal article
- research article
- Published by Taylor & Francis in Current Eye Research
- Vol. 2 (4) , 271-275
- https://doi.org/10.3109/02713688209011630
Abstract
A new method was developed for the conjugation of rat lens glutathione by 1-chloro-2,4-dinitrobenzene (CDNB). This reaction is catalyzed by glutathione S-transferase present in the lens; 1 ml of 1 mM CDNB per 2 rat lenses conjugates > 95% of the lens GSH [reduced glutathione] in 30 min at 37.degree. C. Lenses incubated with 1 mM CDNB for 30 min, followed by incubation in the culture medium without CDNB, remained apparently clear for up to 24 h. The CDNB-treated lenses were more susceptible to protein precipitation (cataract formation) when challenged with oxidants such as H2O2 and superoxide anions.This publication has 22 references indexed in Scilit:
- Defense system of the lens against oxidative damage: Effect of oxidative challenge on cataract formation in glutathione peroxidase deficient-acatalasemic miceExperimental Eye Research, 1980
- Cross-Linking of Lens Crystallins in a Photodynamic System: A Process Mediated by Singlet OxygenScience, 1980
- Glutathione transferase from human erythrocytesArchives of Biochemistry and Biophysics, 1978
- Use of hydroperoxides in the studies of glutathione metabolism in rat lensExperimental Eye Research, 1976
- Role of sulfhydryl groups in the formation of a hereditary cataract in the ratExperimental Eye Research, 1975
- On the presence and mechanism of formation of heavy molecular weight aggregates in human normal and cataractous lensesExperimental Eye Research, 1973
- Metabolism of glutathione in the lensExperimental Eye Research, 1971
- Metabolism of red cell glutathioneExperimental Eye Research, 1971
- Effect of methyl phenyldiazenecarboxylate (azoester) on lens membrane functionExperimental Eye Research, 1970
- Selected Topics in Ophthalmic BiochemistryArchives of Ophthalmology (1950), 1964