In vitrotransformation ofDiplostomum spathaceum(Digenea) cercariae and short term maintenance of post-penetration larvaein vitro

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Abstract
Optimum conditions for the in vitro transformation of Diplostomum spathaceum cercariae have been established by which large numbers of post-penetration larvae (diplostomules) can be produced; short-term maintenance of diplostomules in vitro is described. Experimental studies on this migratory larval stage may now be possible.