Cloning and functional characterization of the human sodium‐dependent vitamin C transporters hSVCT1 and hSVCT2
Open Access
- 1 November 1999
- journal article
- Published by Wiley in FEBS Letters
- Vol. 460 (3) , 480-484
- https://doi.org/10.1016/s0014-5793(99)01393-9
Abstract
Two sodium‐dependent vitamin C transporters, hSVCT1 and hSVCT2, were cloned from a human kidney cDNA library. hSVCT1 had a 1797 bp open reading frame encoding a 598 amino acid polypeptide. The 1953 bp open reading frame of hSVCT2 encoded a 650 amino acid polypeptide. Using a Xenopus laevis oocyte expression system, both transporters were functionally expressed. By Eadie‐Hofstee transformation the apparent K m of hSVCT1 for ascorbate was 252.0 μM and of hSVCT2 for ascorbate was 21.3 μM. Both transporters were sodium‐dependent and did not transport dehydroascorbic acid. Incubation of oocytes expressing either transporter with phorbol 12‐myristate 13‐acetate (PMA) inhibited ascorbate transport activity. Availability of the human transporter clones may facilitate new strategies for determining vitamin C intake.Keywords
This publication has 18 references indexed in Scilit:
- A family of mammalian Na+-dependent L-ascorbic acid transportersNature, 1999
- Absorption, transport, and disposition of ascorbic acid in humansThe Journal of Nutritional Biochemistry, 1998
- Gapped BLAST and PSI-BLAST: a new generation of protein database search programsNucleic Acids Research, 1997
- Glucose Transporter Isoforms GLUT1 and GLUT3 Transport Dehydroascorbic AcidJournal of Biological Chemistry, 1997
- Reactive Oxygen-Mediated Protein Oxidation in Aging and DiseaseChemical Research in Toxicology, 1997
- Purification, cloning and expression of dehydroascorbic acid-reducing activity from human neutrophils: identification as glutaredoxinBiochemical Journal, 1996
- Vitamin C pharmacokinetics in healthy volunteers: evidence for a recommended dietary allowance.Proceedings of the National Academy of Sciences, 1996
- Variation in the Expression and/or Phosphorylation of the Human Low Molecular Weight Stress Protein during in Vitro Cell DifferentiationJournal of Biological Chemistry, 1995
- Dehydroascorbate reductionJournal of Bioenergetics and Biomembranes, 1994
- [14]Xenopus oocyte microinjection: From gene to proteinPublished by Elsevier ,1992