Antibiotic Susceptibility of Legionella pneumophila Philadelphia-1 in Cultured Guinea-Pig Peritoneal Macrophages

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Abstract
The effect of antimicrobial agents on the intracellular multiplication of L. pneumophila in cultured guinea pig peritoneal macrophages was measured. .beta.-Lactam antibiotics at concentrations 5-400 times the MIC [minimum inhibitory concentration] in vitro did not inhibit the intracellular growth of the organism. Gentamicin inhibited the growth considerably but failed to eliminate the organism from the phagocytic mixture. Chloramphenicol or tetracycline at 10 .mu.g/ml (40 or 5 times the MIC in vitro, respectively) did not eliminate the organism. At a higher concentration (30 .mu.g/ml) these drugs eliminated the bacterium from the mixture. Only erythromycin and rifampin were effective in killing the organism at very low concentration (1 .mu.g/ml). Intracellular multiplication of L. pneumophila was observed clearly by light microscopy using Wright-Giemsa staining.

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