Spot Analysis for Glycoprotein Determination in the Nanogram Range
- 1 January 1981
- journal article
- research article
- Published by Walter de Gruyter GmbH in Hoppe-Seyler´s Zeitschrift Für Physiologische Chemie
- Vol. 362 (2) , 1427-1434
- https://doi.org/10.1515/bchm2.1981.362.2.1427
Abstract
A flexible method of glycoprotein determination, with .mu.l-volumes, using spot analysis on cellulose acetate layers, is described. With [Aspergillus niger] glucose oxidase [EC 1.1.3.4] as an example of a glycoprotein and fluorescein isothiocyanate-labeled Concanavalin A, a sensitivity of 10 ng is reached; in combination with horseradish peroxidase [EC 1.11.1.7] 1 ng of glucose oxidase can be detected. A simultaneous determination of protein and glycoprotein with one single spot of 0.5, 1 or 2 .mu.l of a glycoprotein solution can be performed. The method is independent of many common external influences, e.g., dodecyl sulfate, Triton X-100, Nonidet P40, mercaptoethanol and desoxycholate. Only pretreatment of the glycoprotein with urea decreases the sensitivity.This publication has 2 references indexed in Scilit:
- Spot Analysis for Glycoprotein Determination in the Nanogram RangeHoppe-Seyler´s Zeitschrift Für Physiologische Chemie, 1981
- A Simple, Versatile, Sensitive and Volume-Independent Method for Quantitative Protein Determination which is Independent of Other External InfluencesHoppe-Seyler´s Zeitschrift Für Physiologische Chemie, 1979