Incorporation of 7-deaza dGTP during the amplification step in the polymerase chain reaction procedure improves subsequent DNA sequencing
- 1 January 1990
- journal article
- research article
- Published by Taylor & Francis in DNA Sequence
- Vol. 1 (2) , 137-140
- https://doi.org/10.3109/10425179009016041
Abstract
An improved method for sequencing human genomic DNA amplified by the polymerase chain reaction (PCR) procedure is described. The portion of the genome investigated is the 383 nucleotide-long exon 2 of the human antithrombin III gene. Incorporation of the analogue of dGTP, 7-deaza-2′-deoxyguanosine-5′-triphosphate, during the amplification of exon 2 by PCR allowed for the elimination of recurrent artifacts obtained during sequencing of the amplified ONA by the dideox-yribonucleotide chain termination method.Keywords
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