Nucleotide sequence of the transforming gene of simian sarcoma virus.

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Abstract
The sequence of the transforming region of simian sarcoma virus (SSV) was determined by using molecularly cloned viral DNA. This region encompassed the 1.0-kilobase pair woolly monkey cell-derived insertion sequence, v-sis, and flanking simian sarcoma-associated viral (SSAV) sequences. A 675-nucleotide-long open reading frame commenced 19 nucleotides within the SSAV sequences to the left of the v-sis helper viral junction and terminated within v-sis itself. Possible promoter and acceptor splice signals were detected in helper viral sequences upstream from this open reading frame and potential polyadenylylation sites were identified downstream both within v-sis and in helper viral sequences beyond v-sis. The recombinational event that led to the generation of SSV occurred in the middle of 2 functional codons, indicating that SSAV provided the regulatory elements for transcription and the initiation codon for translation of SSV cell-derived transforming sequences.