Cell fractions and enzymatic activities of Ureaplasma urealyticum

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Abstract
The localization of some enzymic activities in cell fractions of U. urealyticum was studied. A quantitative evaluation of the effectiveness of several cell lysis procedures was obtained by using labeled membranes and sucrose density gradient centrifugation. Ultrasonic treatment was the most effective procedure for lysing the cells, but digitonin and osmotic shock caused the lysis of only 70 and 50% of the cells, respectively. The localization of selected enzymes in Ureaplasma cells resembled that found in other Mycoplasma spp. ATPase, RNase, DNase and p-nitrophenylphosphatase activities were located exclusively in the membrane fraction, but urease and L-histidine ammonia-lyase were located in the cytoplasm.