Effect of Cross-Immunization on Monotypic Antibody Responses to Herpes Simplex Virus Types 1 and 2
Open Access
- 1 April 1976
- journal article
- research article
- Published by Oxford University Press (OUP) in The Journal of Immunology
- Vol. 116 (4) , 898-903
- https://doi.org/10.4049/jimmunol.116.4.898
Abstract
New Zealand White rabbits were immunized subcutaneously with partially purified UV-inactivated preparations of herpes simplex virus (HSV-1 or HSV-2) in complete Freund's adjuvant. After the initial immunizations, designated groups of animals received additional amounts of either HSV-1 or HSV-2 at 35-day intervals. Sera were absorbed with lysates of cells infected with heterotypic virus and the residual monotypic antibodies were detected by 51Cr-release assay using HSV-infected target cells. A positive correlation was found between the ratio of neutralizing antibodies to HSV-1 and HSV-2 (II/I index) and the content of monotypic antibodies. Results showed that production of monotypic antibodies to HSV-1 and HSV-2, under the conditions employed, was independent of previous exposure to HSV.This publication has 0 references indexed in Scilit: