Herpesvirus saimiri (HVS) was isolated from peripheral lymphocytes of 16 of 20 squirrel monkeys (Saimiri sciureus) recently imported from the region of Leticia, Amazonas, Colombia. Sera of all 20 animals (including juveniles as young as 1½ years of age) had antibodies to HVS. A group of 10 female squirrel monkeys, captive for more than 1 year and also shown to be HVS carriers, were examined over 5 months for oral excretion of HVS. Virus was consistently isolated from oropharyngeal secretions of 9 monkeys. Filtration studies showed that at least some HVS present in the oropharyngeal secretions was cell free.