Spin Immunoassay of Urinary Testosterone

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Abstract
A spin-labeled derivative of testosterone [T] was prepared by coupling testosterone-3-(O-carboxymethyl)oxime with 3-aminomethyl-2,2,5,5-tetramethyl-1-oxylpyrrolidine and used to develop a method for measuring testosterone glucuronide [TG] in human male urine. The samples were brought to pH 6.8 and incubated for 24 h with .beta.-glucuronidase. The released steroid was extracted by hexane-ethyl ether (4:1) and measured by spin immunoassay (SIA). With a 1 ml urine sample, the ultimate sensitivity of the assay was 18 ng of T. T levels were determined in 8 normal male urine samples simultaneously by SIA and radioimmunoassay. The coefficient of correlation was 0.986. The method was speedy and simple, but not sensitive enough to measure TG in human female urine.