A murine cell possessing a dominant mutation affecting the regulation of interferon production: Characterization by intraspecific hybrids
- 1 November 1978
- journal article
- Published by Springer Nature in Somatic Cell and Molecular Genetics
- Vol. 4 (6) , 677-697
- https://doi.org/10.1007/bf01543158
Abstract
Using intraspecific hybrids, we have demonstrated the dominant nature of two phenotypic markers present in a mutant mouse 3T6 cell line, designated 3T6-V r B2. These are, resistance to virus infection (V r )and semiconstitutive synthesis of interferon (IF sc ).Hybrids were formed by polyethylene glycol-mediated fusion between 3T6-V r B2, or its parent 3T6, and 2TG0-13, a triply marked derivative of mouse 3T3 cells. When tested for the V r marker, 3T6-V r B2 ×2TG0-13 hybrid clones displayed a level of resistance to virus infection which was equal to or greater than that of 3T6-V r B2. Similarly, when tested for the IF sc marker, these hybrid clones were found to possess the capacity to confer an interferon-induced antiviral state in mouse L 929 cells upon cocultivation. By comparison, clones derived from 3T6 × 2TG0-13 fusions produced high levels of virus and failed to confer an interferon-induced antiviral state in L 929 cells.Keywords
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