Electrophoretic Migration of Serum Lipoproteins in Starch Gel.

Publisher Website
Google Scholar
Abstract
Normal human serum lipoproteins were separated by starch granule electrophoresis according to the method of H. Kunkel and by preparative ultracentrifugation. The isolated fractions: alpha-1, alpha-2 and beta lipoproteins as well as the low (less than 1.063) and high (less than 1.21) density lipoproteins were submitted to zone electrophoresis in starch gel according to the technique of O. Smithies. The beta and alpha-2 lipoproteins separated by starch granule electrophoresis and the low density lipoproteins obtained by ultracentrifugation migrated in starch gel close to the origin and formed a plateau. The alpha-1 as well as the high density lipoproteins migrated in the post-albumin region and formed a peak which had a rapidly rising and gradually falling limb. Full serum gave a plateau close to the origin and a peak in the post-albumin region. These patterns were seen constantly only after the coloring agent (oil red O) was rendered free from protein staining components.

This publication has 0 references indexed in Scilit: