Evidence of an anti‐A1 inhibited by EDTA

Abstract
EDTA is added to some commercial A and B cells as a means of preventing hemolysis in serum testing. Unexpected results in serum testing have been reported with EDTA-dependent agglutinins, but not with agglutinins inhibited by EDTA. A serum sample was found to contain an anti-A1 undetected by serum testing, but resulting in incompatible crossmatches. Studies indicated that reactivity was inhibited by the EDTA present in the diluent of the A reagent red cells.