Matrix Solid-Phase Dispersion Extraction and Liquid Chromatographic Determination of Ivermectin in Bovine Liver Tissue

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Abstract
A method for the isolation and liquid chromatographic (LC) determination of the antiparasitic drug ivermectin in bovine liver is presented. Liver samples (0.5 g) are blended with 2 g C18 (octadecylsilylderivatized silica packing material). A column made from the C18liver matrix is washed with 3 mL hexane, then the ivermectin is eluted with methylene chloride-ethyl acetate (3 + 1). After purification by alumina solid-phase extraction, the ivermectin is derivatized and analyzed by LC with fluorescence detection. The overall recovery of ivermectin added to liver was 74.6%. The lowest level validated in tissue by the method was 10 ppb, and the limit of detection was 1 ppb. This method and a classical extraction method gave comparable results for a liver sample that contained incurred ivermectin residues. The method uses small volumes of solvents, has a limited number of sample manipulations, and does not require solvent partitioning or backwashing of extracts. These characteristics make this method attractive when compared to classical isolation procedures for ivermectin.

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