Trinucleotide phosphoramidites: ideal reagents for the synthesis of mixed oligonucleotides for random mutagenesis
- 1 January 1994
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 22 (25) , 5600-5607
- https://doi.org/10.1093/nar/22.25.5600
Abstract
Trinucleotide phosphoramidites representing codons for all 20 amino acids have been prepared and used in automated, solid-phase DNA synthesis. In contrast to an earlier report, we show that these substances can be used to introduce entire codons into oligonucleotides in excess of 98% yield, and are ideal reagents for the synthesis of mixed oligonucleotides for random mutagenesis.Keywords
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