Structural Studies on the Anti-tumor Active Polysaccharides from Coriolus versicolor (Basidiomycetes). I. Fractionation with Barium Hydroxide

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Abstract
A polysaccharide mixture was extracted from the mycelium of C. versicolor with hot water and precipitated with ethanol. Fractionation with increasing concentrations of barium hydroxide solution and then with a cetyltrimethylammonium hydroxide solution afforded 5 fractions, among which a fraction precipitating with 0.3 N barium hydroxide solution had a marked antitumor activity against sarcoma-180 implanted in mice. This fraction was composed of .beta.-D-glucan and a smaller amount of protein, which could not be separated from the glucan moiety by any of the Sevag method, precipitation with trifluorotrichloroethane, digestion with pronase, or chromatography on a column packed with molecular sieves. On hydrolysis, this fraction gave D-glucose as the only carbohydrate component together with common amino acids. A .beta.-glycosidic linkage was suggested from a low value of its specific optical rotation.

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