Propylene and Ethylene Glycol as Solvents for Sudan IV and Sudan Black B

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Abstract
Propylene or ethylene glycol is recommended as a solvent for Sudan IV and Sudan black B to replace the commonly used alcohol-acetone mixtures for general lipid staining in tissue sections. Either glycol is used as a dehydrating agent, dye solvent, and differentiating solution. They offer the advantages of a stable solution, inert with respect to solubilities of lipid material in it, and excellent control of differentiation without loss of dye from lipid particles. Sections remain pliable and are not shrunken by the glycols. Counterstains may be used after staining with Sudan IV but are generally not necessary after staining with Sudan black B. With the use of propylene glycol as a solvent, Sudan IV appears to equal the staining ability of Sudan black B as regards the type of lipid material detected, and the choice of dye to be used would depend on the color contrast desired.

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