Sulfated N‐linked carbohydrate chains in porcine thyroglobulin
- 5 December 1988
- journal article
- Published by Wiley in FEBS Letters
- Vol. 241 (1-2) , 246-250
- https://doi.org/10.1016/0014-5793(88)81070-6
Abstract
N-linked carbohydrate chains of porcine thyroglobulin were released by the hydrazinolysis procedure. The resulting mixture of oligosaccharide-alditols was fractionated by high-voltage paper electrophoresis, the acidic fractions were further separated by high-performance liquid chromatography on Lichrosorb-NH2, and analyzed by 500-MHz 1H-NMR spectroscopy and, partially, by permethylation analysis. Of the acidic oligosaccharide-alditols, the following sulfated carbohydrate chains could be identified:Keywords
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