SUSCEPTIBILITY OF VARIOUS CELL-CULTURE SYSTEMS TO BOVINE VIRAL DIARRHEA VIRUS

  • 1 January 1985
    • journal article
    • research article
    • Vol. 8  (1) , 17-22
Abstract
A comparative study was carried out on the susceptibility of primary bovine embryo kidney (pBEK) cell cultures and those of 3 other cell culture systems, i.e., primary calf testicle (pCT) cells and 2 cell lines, 1 of which originated from bovine embryonic trachea (EBTr) and the other from buffalo (IMR-31) to bovine viral diarrhea (BVD) virus. The virus titers obtained in the 3 culture systems under study did not differ significantly from those of pBEK cells. The growth curve study demonstrated that the pCT cells, and the 2 cell lines, were similar to the pBEK cultures in their susceptibility to the virus. Accordingly, pCT, EBTr and IMR-51 cell cultures possess the general requirements for use in studies on the BVD virus. However, since the cytopathic effects (CPE) produced in the EBTr and the IMR-51 cell lines did not display any distinctive characteristics typical of BVD virus-induced CPE, these 2 cell lines should not be used for isolation of the virus from specimen material.

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