Production of high-frequency embryogenic calli from integuments of immature seeds of Carica Papaya , L.

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Abstract
Tissue culture conditions that enable production in Carica papaya L. of high frequency embryogenic calli on the internal integuments of immature seeds are defined: The explants have to be removed three or four months after anthesis and cultured on the three monthly successive media: Ml-1 (2,4-D 9 µM), M2 (2,4-D 0.09 µM + KIN 1 µM), M3 (M2 + ABA 0.5 µM). A histocytological study during culture proved that friable calli capable of producing embryos were of maternal origin. This procedure thus permits the cloning of adult plants.

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