Nonenzymatic radiolabeling of protein by 32P‐containing nucleotides
- 6 January 1986
- journal article
- Published by Wiley in FEBS Letters
- Vol. 194 (2) , 305-308
- https://doi.org/10.1016/0014-5793(86)80106-5
Abstract
We report a nonenzymatic reaction which results in the radiolabeling of proteins by 32P‐containing nucleoside triphosphates. The labeling reaction does not require any cofactors, but is greatly enhanced by the presence of alcohols. Even under optimal conditions, less than 1% of the protein molecules undergo modification. This nonspecific labeling represents a serious artifact which may become significant in systems involving low levels of specific labeling, such as photoaffinity labeling. Since the reaction is not limited to specific proteins, this may, however, provide a simple and rapid procedure for the preparation of labeled proteins.Keywords
This publication has 7 references indexed in Scilit:
- Amplification and isolation of E. coli nusA protein and studies of its effects on in vitro RNA chain elongationBiochemistry, 1984
- Topography of transcription: path of the leading end of nascent RNA through the Escherichia coli transcription complex.Proceedings of the National Academy of Sciences, 1983
- In Vivo Chemical Modification of Proteins (Post-Translational Modification)Annual Review of Biochemistry, 1981
- Phosphorylation of nuclear proteins in rat regenerating liverBiochemistry, 1977
- Identification of a gamma-glutamyl methyl ester in bacterial membrane protein involved in chemotaxis.Journal of Biological Chemistry, 1977
- The rapid, simple and improved preparation of high specific activity α-[32P]dATP and α-[32p]ATPNucleic Acids Research, 1977
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970