Fertilization and pregnancies following intracytoplasmic injection of testicular spermatozoa
- 1 October 1995
- journal article
- Published by Springer Nature in Journal of Assisted Reproduction and Genetics
- Vol. 12 (9) , 627-631
- https://doi.org/10.1007/bf02212587
Abstract
Intracytoplasmic injection (ICSI) with testicular sperm was performed in 16 couples. All men had ductal obstruction and failed previous attempts of epididymal sperm microaspiration.Testis tissue was obtained by excisional biopsies and incubated in HEPES buffered EBSS medium over 24 h at 37 degrees C. Motile sperm (Grade 1 to 2) were recovered in 13 patients and fertilized a total of 62 oozytes. Four pregnancies were achieved.One healthy boy and two girls (twin pregnancy) were born.The ongoing pregnancies revealed no fetal abnormalities on ultrasound scanning.Keywords
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