Root reductant release as a measure of sorghum cultivar iron efficiency

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Abstract
Measurement of root reductant levels developed during plant Fe stress was tested as a possible assay for sorghum cultivar Fe-efficiency screening. Iron-stressed sorghum as shown to release reductants into CaCO3 buffered nutrient solution; however, considerably more plants could be tested by extracting reductants from excised roots of Fe-stressed sorghum in 35 ml of pH 3 nutrient solution and 1 m M glucose. An Fe-efficient cultivar, RTx2536, and an Fe-inefficient cultivar, BTx378, could be separated by measurement of reductants released into CaCO3 buffered nutrient solution and by an excised root extraction method; however, neither method was as effective as visual rating methods.