185W-labelled nitrate reductase from Chlorella1

Abstract
By adding 185W-tungstate to a Chlorella culture, it has been possible to incorporate this metal into the nitrate reductase complex. The W-labelled enzyme was completely inactive as nitrate reductase, but maintained unaffected its diaphorase activity. In vivo incorporation of tungsten into the enzyme was competitively hindered by molybdenum.

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