The Ultracentrifugation of Soluble Cytochromes

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Abstract
A particulate prepn. of mammalian heart muscle dissolved with the aid of Na desoxycholate was studied in the ultracentrifuge. There is evidence that the cyto chromes b and c and oxidase are present in this prepn. After ultracentrifugation the soln. was transferred to a microcurette of the spectrophotometer, reduced with Na2S2O4, and covered with mineral oil for spectral analysis. These expts. revealed that cytochrome b is the most rapidly sedimenting component, cytochrome oxidase is intermediate, and that cytochrome c sediments most slowly.
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