Biotransformation of the Naturally Occurring Lignan (-)-Arctigenin in Mammalian Cell Lines Genetically Engineered for Expression of Single Cytochrome P450 Isoforms
- 1 October 1994
- journal article
- research article
- Published by Georg Thieme Verlag KG in Planta Medica
- Vol. 60 (05) , 441-444
- https://doi.org/10.1055/s-2006-959528
Abstract
V79 Chinese hamster cells genetically engineered for stable expression of single forms of rat cytochromes P450 1A1, P450 1A2, and P450 2B1, and human P450 1A2 were used to analyse the metabolism of (-)-arctigenin. Concentrations of metabolites formed in the medium were determined by TLC, HPLC, GC, and GC-MS. Only in the cell line expressing rat P450 2B1 was (-)-artigenin metabolized to a significant extent. The lignan was converted by O-demethylation to (-)-3′-O-demethylarctigenin.Keywords
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