A Procedure for Differential Staining of Cartilage and Bone in Whole Formalin-Fixed Vertebrates
- 1 January 1976
- journal article
- research article
- Published by Taylor & Francis in Stain Technology
- Vol. 51 (2) , 131-134
- https://doi.org/10.3109/10520297609116684
Abstract
This paper describes a modification of the Simons and Van Horn (1971) procedure for rendering cartilage blue, bone red, and soft tissue translucent or transparent in whole vertebrate specimens. Alcian blue and alizarin red S are used to stain cartilage and bone respectively. In our procedure formalin is used as a fixative. This is a significant modification because formalin is the common fixative for museum specimens. This clearing and staining procedure is thus readily applicable to comparative studies in anatomy, embryology and systematic zoology.This publication has 5 references indexed in Scilit:
- Selective Skeletal Staining in Whole Chicken Embryos; A Rapid Alcian Blue TechniqueStain Technology, 1970
- Toluidine Blue-Alizarin Red S Staining of Cartilage and Bone in Whole-Mount Skeletons in VitroStain Technology, 1965
- Two Organic Fixatives for Acid MucopolysaccharidesStain Technology, 1956
- Alizarin Red S and Toluidine Blue for Differentiating Adult or Embryonic Bone and CartilageStain Technology, 1941