Separation and determination of cardiac steroids by high-pressure liquid chromatography.
- 31 December 1975
- journal article
- research article
- Published by Pharmaceutical Society of Japan in CHEMICAL & PHARMACEUTICAL BULLETIN
- Vol. 24 (12) , 2995-3000
- https://doi.org/10.1248/cpb.24.2995
Abstract
A method for the simultaneous analysis of cardiac steroids by high-pressure liquid chromatography was developed. Reversed phase chromatography on a .mu.-Bondapak C18 column was particularly effective for the separation of 16 bufadienolides and 9 cardenolides. Determination of the principal bufogenins in the fresh venom of the Japanese toad was carried out by the internal standard method. The relationship between chemical structures and retention values was also discussed.This publication has 2 references indexed in Scilit:
- Studies on steroidsJournal of Chromatography A, 1976
- Occurrence of bufalitoxin, cinobufotoxin and their homologs in Japanese toad.CHEMICAL & PHARMACEUTICAL BULLETIN, 1976