Fluorometric determination of phenylpyruvic acid with 1,4-dimethyl-3-carbamoylpyridinium chloride.
- 31 December 1986
- journal article
- research article
- Published by Pharmaceutical Society of Japan in CHEMICAL & PHARMACEUTICAL BULLETIN
- Vol. 35 (9) , 3746-3749
- https://doi.org/10.1248/cpb.35.3746
Abstract
A fluorometric method for the determination of phenylpyruvic acid (PPA) has been established. Benzaldehyde formed from PPA by alkaline decomposition is quantified by means of its fluorogenic reaction with 1, 4-dimethyl-3-carbamoylpyridinium chloride. PPA can be determined in the range of 0.1-3 nmol/50 μl with the relative standard deviation of 4.0-4.1%. About 70% recovery was obtained when a urine sample (0.1 ml) added with 5 nmol of PPA was extracted and then determined by the proposed method. The method should be useful for the biochemical diagnosis of phenylketonuria.Keywords
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