We have compared two media (Tryptone Soya Nalidixic Acid Agar and Tryptone Soya Gum Base Nalidixic Acid Medium) for the isolation of L. monocytogenes from pathological and environmental specimens. These media were used in conjunction with Henry's Oblique Light System. We found that the gum based medium gave the better results. The medium is uniformly transparent, partially suppressive to unwanted organisms and allows colonies of Listeria to be readily identified.