Interaction between the Exocellular DD‐Carboxypeptidase‐Transpeptidase from Streptomyces R61, Substrate and β‐Lactam Antibiotics
- 1 September 1975
- journal article
- Published by Wiley in European Journal of Biochemistry
- Vol. 57 (2) , 353-359
- https://doi.org/10.1111/j.1432-1033.1975.tb02308.x
Abstract
The interaction between the exocellular DD‐carboxypeptidase‐transpeptidase of Streptomyces R61 and β‐lactam antibiotics is a multistep process during which a rather stable enzyme · antibiotic complex is formed. This mechanism of interaction is compatible with Lineweaver‐Burk plots that are typical of a competitive inhibition of the hydrolysis of the peptide donor by the antibiotic. In fact, however, the same Lineweaver‐Burk plots can be obtained on the basis of a non‐competitive type of inhibition. At present, a choice between the two models cannot be made.Keywords
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