Reactivity to IgE-dependent histamine-releasing factor is due to monomeric IgE
- 1 July 2000
- Vol. 55 (7) , 653-657
- https://doi.org/10.1034/j.1398-9995.2000.00573.x
Abstract
IgE-dependent histamine-releasing factor (HRF) can distinguish between IgE+ and IgE-. In contrast to IgE-, IgE+ sensitizes basophils to release histamine in response to HRF. But we do not know what particular feature distinguishes IgE+ from IgE-. The objective was to investigate the hypothesis that IgE+ is polymeric IgE. IgE+ plasma was separated by size-exclusion chromatography. The basophil-sensitizing capacity of the fractions was analyzed in response to HRF produced by mononuclear cells. We showed that monomeric IgE sensitized basophils to release histamine in response to HRF and to house-dust mite, whereas no enhanced reactivity was found in the fractions containing polymeric IgE. HRF reacts with monomeric IgE, and not (exclusively) with polymeric IgE.Keywords
This publication has 17 references indexed in Scilit:
- Degranulation of human basophils bypicomolar concentrations of IL–3, IL–5, orgranulocyte-macrophage colony-stimulating factor☆☆☆★★★Journal of Allergy and Clinical Immunology, 1998
- Mouse/human chimeric IgGl and IgG4 antibodies directed to the house dust mite allergen Der p 2: use in quantification of allergen specific IgGClinical and Experimental Allergy, 1997
- Histamine-releasing activity in supernatants of mononuclear cells: Contribution of monocyte chemotactic protein-1 activity compared with IgE-dependent activityJournal of Allergy and Clinical Immunology, 1996
- Lectins do not distinguish between heterogenous IgE molecules as defined by differential activity of an IgE-dependent histamine releasing factorJournal of Allergy and Clinical Immunology, 1996
- Expression of Novel Secreted Isoforms of Human Immunoglobulin E ProteinsPublished by Elsevier ,1996
- Alternative RNA of Epsilon Transcripts Produces mRNAs Encoding Two Membrane and Four Secreted IgE IsoformsInternational Archives of Allergy and Immunology, 1995
- In vitro basophil histamine-releasing activity of circulating IgG1 and IgG4 autoanti-IgE antibodies from asthma patients and the demonstration that anti-IgE modulates allergen-induced basophil activationClinical and Experimental Allergy, 1994
- Discrepancies between the skin test and IgE antibody assays: Study of histamine release, complement activation in vitro, and occurrence of allergen-specific IgGJournal of Allergy and Clinical Immunology, 1988
- Interaction of Human Polyclonal IgE and IgG from Different Species with Protein A from Staphylococcus aureus: Demonstration of Protein‐A‐reactive Sites Located in the Fab2 Fragment of Human IgGScandinavian Journal of Immunology, 1980
- Interaction of Polyclonal Human IgE with Protein‐A from Staphylococcus aureusImmunological Reviews, 1978