Selective Isolation of the Entomopathogenic Fungi Beauveria bassiana and Metarhizium anisopliae from an Artificial Potting Medium
- 1 June 1986
- journal article
- research article
- Published by Walter de Gruyter GmbH in The Florida Entomologist
- Vol. 69 (2) , 285-292
- https://doi.org/10.2307/3494930
Abstract
A basal medium consisting of oatmeal agar was superior to seven other media for isolation of Metarhizium anisopliae and Beauveria bassiana from an artificial potting medium. A rate of 0.55 g/l of dodine and 5 mg/l of chlortetracycline allowed optimum recovery of B. bassiana alone. Decreasing the amount of dodine to 0.46 g/l and adding 0.38 g benomyl allowed recovery of both entomopathogens at high frequencies in the absence of most other fungi. Addition of 10 mg/l crystal violet aided in visibility of the target fungi without affecting recovery frequencies. Exposure of culture plates to as little as 2 .mu.mol m-2s-1 light delayed observation of colonies of both entomopathogens up to 7 days compared to cultures incubated in the dark.This publication has 4 references indexed in Scilit:
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