Methylase-limited partialNotI cleavage for physical maping of genomic DNA
- 1 January 1990
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 18 (11) , 3287-3291
- https://doi.org/10.1093/nar/18.11.3287
Abstract
Partial cleavage of DNA with the restriction endonuclease NotI (5''...GC/GGCCGC...3'') is an important technique for genomic mapping. However, partial genomic cleavage with this enzyme is impaired by the agarose matrix in which the DNA must be suspended. To solve this problem we have purified the blocking methylase M. BspRI (5''...GGmCC...3'') for competition digests with NotI. The resulting methylase-limited partial DNA cleavage is shown to be superior to standard techniques on bacterial genomic DNA.This publication has 19 references indexed in Scilit:
- Inhibition of DNA Binding Proteins by Oligonucleotide-Directed Triple Helix FormationScience, 1989
- Isolation and characterization of a human telomereNucleic Acids Research, 1989
- Controlled partial restriction digestions of DNA by competition with modification methyltransferasesAnalytical Biochemistry, 1989
- Cloning of human telomeres by complementation in yeastNature, 1989
- Molecular cloning of human telomeres in yeastNature, 1989
- Improved control of partial DNA restriction enzyme digest in agarose using limiting concentrations of Mg+ +Nucleic Acids Research, 1989
- Enzymatic cleavage of a bacterial genome at a 10-base-pair recognition site.Proceedings of the National Academy of Sciences, 1989
- Activity of DNA modification and restriction enzymes in KGB, a potassium glutamate bufferGene Analysis Techniques, 1988
- Construction and characterization of mutations in hupB, the gene encoding HU-beta (HU-1) in Escherichia coli K-12Journal of Bacteriology, 1988
- Sequence-Specific Cleavage of Double Helical DNA by Triple Helix FormationScience, 1987