Kupffer cell depletionin vivoresults in preferential elimination of IgG aggregates and immune complexes via specific Fc receptors on rat liver endothelial cells

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Abstract
SUMMARY: In the present study we have investigated the clearance kinetics and tissue distribution of monomeric (m) IgG and soluble aggregates of IgG (AlgG) and immune complexes (IC) in normal and Kupffer cell (KC) depleted rats. In normal rats, clearance of mIgG occurred in a biphasic manner with a first half-life (T) or 36±3 ± 6±3 min and a second T±(T2) of 168±4±4±7 min. AlgG composed of 20-27 IgG molecules per aggregate were cleared significantly faster than mIgG with a TI of 2±5±0±1 min and a T2 of 32±5±5±6 min. KC depletion did not have a significant effect on the clearance rate of mIgG (TI: 33±4 ± 8±9 min; T2: 159±5± 12±5 min), while clearance of AlgG was delayed significantly wilh TI 4±8 ± 0±7 min and T2 41±2 ± 3±2 min. Eight minutes after injection. 77% of AIgG was found in the liver in normal rats while 62% was found in the liver of KC-depleted rats. Double immunofluorescence studies indicated that AIgG in the liver was associated with KC and endothelial cells (EC) in normal rats. In KC-depleted rats, AIgG was strongly associated with EC. A similar staining pattern was observed when IgG-immune IC were administered. The clearance of AIgG in KC-depleted rats was inhibited fully by pre-administration of high concentrations of IgG but not by pretreatment with IgA. asialofetuin (ASFe) or ovalbumin (OVA). Aggregated F(ab')2IgG was cleared with a comparable rate to mIgG from the circulation, again suggesting Fcγ receptor-mediated elimination of AIgG by EC. There was a reduced degradation of AIgG in rats depleted of KC as compared with normal rats. These data suggest binding and degradation of AIgG by EC in vivo.