Properties of the tungsten‐substituted molybdenum formylmethanofuran dehydrogenase from Methanobacterium wolfei
- 31 August 1992
- journal article
- Published by Wiley in FEBS Letters
- Vol. 309 (1) , 78-81
- https://doi.org/10.1016/0014-5793(92)80743-z
Abstract
In Methanobacterium wolfei two formylmethanofuran dehydrogenases are present, one of which is a molybdenum- and the other a tungsten enzyme. We report here that also the ‘molybdenum’ enzyme contained tungsten when the archaeon was grown on molybdenum-deprived medium supplemented with tungstate (1 μM). Unexpectedly the tungsten-substituted molybdenum enzyme was catalytically active and displayed a rhombic EPR signal which was attributed to tungsten by the characteristic 113W splitting.Keywords
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