A technique for revealing tissue embedded within resin blocks

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Abstract
SUMMARY: A technique is described for revealing in fine detail large pieces of tissue still embedded within rough, scratched or partly‐trimmed blocks of epoxy and other resin before sectioning. The pieces of tissue immersed in baths of oil or glycerol and illuminated by an appropriate optical system, may be photographed to provide a guide to subsequent sectioning, aiding the precise location of elements in the sections back to their position in the parent tissue, and for tracing the connections of the element between serial sections. The method has been used by the authors in sectioning for light microscopy, but could equally be used with sections for electron microscopy.

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